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Título : The effect of pasteurization in the expression of bovine milk microRNA
Autor : Abou El Qassim, Loubna
Royo, Luis José
Palabras clave : Biomarcadores
Leche de vaca
Fecha de publicación : 2021
Citación : Abou El Qassim, L.; Royo, L.J. The effect of pasteurization in the expression of bovine milk microRNA. [Póster]. En: Book of Abstracts of the 72nd Annual Meeting of European Federation of Animal Science EAAP The Netherlands: Wageningen Academic Publishers; 2021. p. 637
Resumen : The edaphoclimatic conditions in Asturias (northwest Spain) enable the presence of milk production systems oscillating between farms based on grazing and low consumption of conserved feed and forages as well as a low density of animals per surface, and intensive farms with confined animals, with a higher density, and higher consumption of conserved feed and forages. Identifying milk according to its origin is a useful way to highlight the positive effects of grazing farms on the environment, milk quality, animal welfare, and social aspects. MicroRNAs (miRNAs) are 21-25-nucleotide small RNAs that perform various functions within cells, including gene expression regulation. They can be profiled through microarray, quantitative real-time Polymerase Chain Reaction (RT-qPCR), or sequencing. Their expressions vary according to the genetic context and external factors to the animal (feeding, handling, etc.). Besides, these molecules are resistant to adverse physicochemical conditions, which make them potential biomarkers. In previous studies, a set of miRNAs from raw milk with differential expression according to some ingredients of the diet were determined. However, it is important to evaluate the effect of technological treatments, in milk factories,(pasteurization, fermentation, sterilization, etc.) on these miRNAs. Furthermore, taking into account the potential bioactivity of milk according to its miRNAs content, it is important to assess miRNAs milk content intended for human consumption. In the present study, we analysed the effect of pasteurization on the miRNAs determined as biomarkers. For that purpose, the expression of nine miRNAs was analysed in the same volume of twenty tank milk samples before and after pasteurization. The expression analysis of the different biomarkers in pasteurized and non-pasteurized milk showed that the expression of most miRNAs was lower after pasteurization, although without significant differences (P<0.05). Moreover, pasteurization of raw milk affects differently fat and cell fraction. These results suggest that miRNAs are quite resistant to pasteurization.
URI : http://ria.asturias.es/RIA/handle/123456789/14534
Aparece en las colecciones: Agroalimentación y Ganadería

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