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Título : High-Performance Liquid Chromatography of the Neutral Phenolic Compounds of Low Molecular Weight in Apple Juice
Autor : Suárez Valles, Belén
Santamaría Victorero, J.
Mangas Alonso, Juan J.
Blanco-Gomis, Domingo
Palabras clave : Phenolic compounds
Apple juice
Fecha de publicación : dic-1994
Editorial : American Chemical Society
Citación : Suárez Valles, B.; Santamaría Victorero, J.; Mangas Alonso, J.J.; Blanco Gomis, D. High-Performance Liquid Chromatography of the Neutral Phenolic Compounds of Low Molecular Weight in Apple Juice. Journal of Agricultural and Food Chemistry. 1994; 42 (12): 2732–2736.
Resumen : The neutral phenolic compounds, (+)-catechin, (-)-epicatechin, flavan-3-ols polymers (procyanidins), phloridzin, phloretin xyloglucoside, and the flavonol glycosides (isoquercitrin, hyperin, quercitrin, avicularin, and rutin), in apple juice were extracted with ethyl acetate and separated and quantified by reversed-phase HPLC on a C18 column using an acidified water (pH 2.8)-methanol solvent gradient. A diode array detector was used to evaluate the separation and to identify the phenolic compounds. The recovery obtained for the flavanols and some dihydrochalcones ranged between 93% and 106%. On the contrary, the 30% recovery rate obtained for flavonols shows that ethyl acetate liquid-liquid extraction, usually employed in this type of study, is not recommended for the extraction of these last compounds.
URI : http://ria.asturias.es/RIA/handle/123456789/1681
ISSN : 0021-8561
Aparece en las colecciones: Agroalimentación y Ganadería
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