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Por favor, use este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/2087
Título : Simultaneous Detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, Three Major Fish Pathogens, by Multiplex PCR
Autor : Cerro, A.
Márquez, I.
Guijarro, J. A.
Fecha de publicación : oct-2002
Editorial : American Society for Microbiology
Citación : Del Cerro, A.; Márquez, I.; Guijarro, J. A. Simultaneous Detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, Three Major Fish Pathogens, by Multiplex PCR. Applied and Environmental Microbiology. 2002; 68 (10): 5177-5180.
Resumen : A multiplex PCR assay based on the 16S rRNA genes was developed for the simultaneous detection of three major fish pathogens, Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri. The assay proved to be specific and as sensitive as each single PCR assay, with detection limits in the range of 6, 0.6, and 27 CFU for A. salmonicida, F. psychrophilum, and Y. ruckeri, respectively. The assay was useful for the detection of the bacteria in artificially infected fish as well as in fish farm outbreaks. Results revealed that this multiplex PCR system permits a specific, sensitive, reproducible, and rapid method for the routine laboratory diagnosis of infections produced by these three bacteria.
URI : http://ria.asturias.es/RIA/handle/123456789/2087
ISSN : 0099-2240
Aparece en las colecciones: Agroalimentación y Ganadería
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