Ir a la página de inicio del Gobierno del Principado de Asturias

Datos del Documento

Utilice este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/2088


Título: 9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells
Autores: Gómez, Enrique
Royo Martín, Luis J.
Duque, Paloma
Carneiro, Gustavo
Hidalgo Ordóñez, Carlos O.
Goyache, Félix
Lorenzo, Pedro L.
Álvarez, Isabel
Facal, Nieves
Díez, Carmen
Palabras Claves: Embryo
Ethanol
Viability
Cytoplasmic granule
Fecha Edición: Nov-2003
Editor: WILEY-LISS, INC
Cita Bibliográfica: Gómez, Enrique; Royo, Luis-José; Duque, Paloma; Carneiro, Gustavo; Hidalgo, Carlos; Goyache, Félix [et. al]. 9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells. Molecular Reproduction and Development. 2003; 66 (3): 247-255.
Resumen: The isomer 9-cis of retinoic acid (9-cis-RA) exerts a beneficial effect on bovine in vitro development when added to in vitro maturation (IVM) culture. In the present work, 9-cis-RA 5 nM was found to be stimulatory as opposed to 500 nM (toxic). Cumulus-oocyte complexes (COCs) were treated with the found physiological dose 9-cis-RA 5 nM, and the next determinations performed: (1) relative expression of midkine (MK) and IGF-I, by reverse transcriptase-polymerase chain reaction (RT-PCR), in cumulus-granulosa cells detached from oocytes; (2) cytoplasmic granular migration, by labeling of oocytes with fluoroscein isothiocyanate lectins; and (3) in vitro survival of blastocysts after vitrification and warming. Gene expression of MK was enhanced by 9-cis-RA, but not by 1% ethanol (vehicle). However, we did not detect IGF-I expression, both in dependence on or in the absence of 9-cis-RA acting on cumulus-granulosa cells. The ability of vitrified blastocysts to survive in vitro was not improved by 9-cis-RA. Nevertheless, since only blastocysts obtained from oocytes matured with serum survived, more factors should be considered when evaluating cryopreservation survival. The complete granular migration observed in oocytes matured with 9-cis-RA anticipates the gain in developmental competence of the oocyte, being MK probably involved in this beneficial effect.
URI: https://ria.asturias.es/RIA/handle/123456789/2088
ISSN: 1040-452X
Aparece en las Colecciones:Agroalimentación y Ganadería

Archivos en este documento:



Archivo TamañoFormato
9-cis-retinoic.pdf181,01 kBAdobe PDFVer/Abrir



logo

Todos los documentos en RIA están protegidos por derechos de autor.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - Contacto