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Utilice este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/2104


Título: Use of two replacements of serum during bovine embryo culture in vitro
Autores: Duque, Paloma
González, Enrique
Díaz, Elena
Facal, Nieves
Hidalgo Ordóñez, Carlos O.
Díez, Carmen
Palabras Claves: In vitro produced embryo
Bovine
Serum
Serum replacement
Cell number
Fecha Edición: Feb-2003
Editor: Elsevier
Cita Bibliográfica: Duque, Paloma; González, Enrique; Díaz, Elena; Facal, Nieves; Hidalgo, Carlos; Díez, Carmen. Use of two replacements of serum during bovine embryo culture in vitro. Theriogenology. 2003; 59 (3-4): 889-899.
Resumen: This study evaluated the effect of two commercial serum replacements (Ultroser G and CPSR-3 on in vitro bovine embryo culture. In Experiment 1, zygotes were cultured in SOF+Ultroser G (2, 4 and 6%), SOF+CPSR-3 (2, 4 and 6%), and SOF+5% FCS (control). Blastocyst rates obtained after culturing with Ultroser G were lower than those with FCS. However, blastocyst rates for CPSR-3 were similar to those for serum. In addition, embryos produced in SOF+CPSR-3 had the same proportion inner cell mass number and total cell number as embryos cultured with FCS. In Experiment 2, a combination of serum replacements during different periods showed that treatment before the five-to eight-cell stages had no effect on further embryo development. However, treatments up to the morula stage affected blastocyst formation. The concentration of supplement and the timing of its inclusion in culture markedly affected embryo development. The serum replacement CPSR-3 can supplement embryo culture with blastocyst rates and quality similar to those for serum.
URI: https://ria.asturias.es/RIA/handle/123456789/2104
ISSN: 0093-691X
Aparece en las Colecciones:Agroalimentación y Ganadería
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