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Título : Intrauterine Neospora caninum inoculation of heifers
Autor : Serrano, E.
Ferre, I.
Osoro Otaduy, Koldo
Aduriz, G.
Mateos-Sanz, A.
Martínez, A.
Atxaerandio, R.
Hidalgo Ordóñez, Carlos O.
Ortega-Mora, L. M.
Palabras clave : Neospora caninum
Intrauterine inoculation
Transmission
Heifers
Semen
Fecha de publicación : feb-2006
Editorial : Elsevier
Citación : Serrano, E.; Ferre, I.; Osoro, K.; Aduriz, G.; Mateos-Sanz, A.; Martínez, A.; Atxaerandio, R.; Hidalgo, C. O.; Ortega-Mora, L. M. Intrauterine Neospora caninum inoculation of heifers. Veterinary Parasitology. 2006; 135 (3-4): 197-203.
Resumen : Here, we studied the potential of Neospora caninum tachyzoites to infect heifers when administered in utero by artificial insemination via contaminated semen. Eighteen primiparous cyclic heifers were hormonally synchronized and artificially inseminated. Nine of them, which were inseminated with semen containing 10(7) live N. caninum NC-1 isolate-tachyzoites, reacted with seroconversion and a specific IFN-gamma response. Moreover, N. caninum DNA was demonstrated by a nested-PCR in the blood of all nine heifers and in brain, lungs, liver and uterine horn of several of them. In contrast, nine heifers inseminated with tachyzoite-free semen developed no antibody or IFN-gamma responses, and no parasite DNA was detected in blood or organs. At necropsy, viable embryos were detected in one and six of the infected and non-infected heifers, respectively. No specific Neospora DNA was detected in any of the embryos. This study provides evidence that intrauterine inoculation via contaminated semen cause N. caninum infection in cattle.
URI : http://ria.asturias.es/RIA/handle/123456789/2168
ISSN : 0304-4017
Aparece en las colecciones: Agroalimentación y Ganadería
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