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Título: Acetoacetate and β-d-hydroxybutyrate as energy substrates during early bovine embryo development in vitro
Autores: Gómez, E.
Palabras Claves: Acetoacetate
Fecha Edición: Jul-1997
Editor: Elsevier
Cita Bibliográfica: Gómez, E. Acetoacetate and β-d-hydroxybutyrate as energy substrates during early bovine embryo development in vitro. Theriogenology. 1997; 48 (1): 63-74.
Resumen: We examined the effects of acetoacetate and other metabolic products of fatty acid oxidation on early bovine embryo development. In vitro produced bovine zygotes were cultured in modified-synthetic oviduct fluid medium supplemented with acetoacetate, acetoacetate derivatives, acetyl CoA precursors and lithium chloride. Acetoacetate and all acetoacetate derivatives, with the exception of the ethyl ester, supported in vitro development up to the hatched blastocyst stage at rates similar to that of controls supplemented with lactate/pyruvate. The optimal concentration of acetoacetate in supporting embryo development was 3.6 mM; addition of 1.8 and 3.6 mM lithium chloride did not significantly affect embryo development, while 7.2 mM was inhibitory. Hatched blastocysts cultured with 3.6 mM acetoacetate contained a similar number of cells as the lactate/pyruvate control group. It can be concluded that in vitro produced bovine embryos can develop using ketone bodies as energy substrates, which could be derived in vivo from endogenous lipids.
ISSN: 0093-691X
Aparece en las Colecciones:Agroalimentación y Ganadería
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