Datos del Documento

Por favor, use este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/2504
Título : Identifying the most suitable endogenous control for determining gene expression in hearts from organ donors
Autor : Pérez, Silvia
Royo Martín, Luis J.
Astudillo, Aurora
Escudero, Dolores
Álvarez, Francisco
Rodríguez, Aida
Gómez, Enrique
Otero Guerra, Jesús
Fecha de publicación : dic-2007
Editorial : BioMed Central
Citación : Pérez, Silvia; Royo, Luis J.; Astudillo, Aurora; Escudero, Dolores; Álvarez, Francisco; Rodríguez, Aida; Gómez, Enrique; Otero, Jesús. Identifying the most suitable endogenous control for determining gene expression in hearts from organ donors. BMC Molecular Biology. 2007; 114 (8).
Resumen : BACKGROUND: Quantitative real-time reverse transcription PCR (qRT-PCR) is a useful tool for assessing gene expression in different tissues, but the choice of adequate controls is critical to normalise the results, thereby avoiding differences and maximizing sensitivity and accuracy. So far, many genes have been used as a single reference gene, without having previously verified their value as controls. This practice can lead to incorrect conclusions and recent evidence indicates a need to use the geometric mean of data from several control genes. Here, we identified an appropriate set of genes to be used as an endogenous reference for quantifying gene expression in human heart tissue. RESULTS: Our findings indicate that out of ten commonly used reference genes (GADPH, PPIA, ACTB, YWHAZ, RRN18S, B2M, UBC, TBP, RPLP and HPRT), PPIA, RPLP and GADPH show the most stable gene transcription levels in left ventricle specimens obtained from organ donors, as assessed using geNorm and Normfinder software. The expression of TBP was found to be highly regulated. CONCLUSION: We propose the use of PPIA, RPLP and GADPH as reference genes for the accurate normalisation of qRT-PCR performed on heart tissue. TBP should not be used as a control in this type of tissue.
URI : http://ria.asturias.es/RIA/handle/123456789/2504
ISSN : 1471-2199
Aparece en las colecciones: Agroalimentación y Ganadería
Open Access DRIVERset

Archivos en este documento:
Fichero Tamaño Formato  
Archivo.pdf612.35 kBAdobe PDFVer/Abrir
Mostrar el registro Completo

Ver estadísticas del documento

Este documento está sujeto a una licencia Creative Commons: Licencia Creative Commons Creative Commons