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Por favor, use este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/2521
Título : Cryopreservation of Brown Bear Skin Biopsies
Autor : Caamaño, José N.
Rodríguez, A.
Muñoz, M.
Frutos, C.
Díez, C.
Gómez, E.
Fecha de publicación : 2008
Citación : Caamaño, J. N.; Rodríguez, A.; Muñóz, A.; De Frutos, C.; Díez, C.; Gómez, E. Cryopreservation of Brown Bear Skin Biopsies. Cell Preservation Technology. 2008; 6 (1): 83-86.
Resumen : Genetic resource banks and assisted reproductive technologies support the conservation of endangered or threatened species. In this study we assessed two procedures to cryopreserve skin biopsies from live brown bears. Skin biopsies were taken from six live, anesthetized brown bears. Single biopsies (n = 3) of each animal were cut into small pieces and assigned to one of the three experimental groups: freezing, vitrification, or untreated fresh. There were no differences on cell attachment. However, both freezing and fresh culture allowed for higher cell proliferation (p < 0.05) and less days to reach 70% to 80% confluence (p < 0.03) than vitrification. Skin biopsies from brown bears can be preserved long term, allowing fibroblasts to proliferate in culture. Slow freezing was effective to cryopreserve skin biopsies from brown bears.
URI : http://ria.asturias.es/RIA/handle/123456789/2521
ISSN : 1538-344X
Aparece en las colecciones: Agroalimentación y Ganadería
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