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Título: Conventional pluripotency markers are unspecific for bovine embryonic-derived cell-lines
Autores: Muñoz, M.
Rodríguez, A.
Frutos, C.
Caamaño, José N.
Díez, C.
Facal, Nieves
Gómez, E.
Palabras Claves: Bovine embryos
Embryonic stem cells
Cell surface markers
Fecha Edición: Jun-2008
Editor: Elsevier
Cita Bibliográfica: Muñóz, M.; Rodríguez, A.; De Frutos, C.; Caamaño, J. N.; Díez, C.; Facal, N.; Gómez, E. Conventional pluripotency markers are unspecific for bovine embryonic-derived cell-lines. Theriogenology. 2008; 69 (9): 1159-1164.
Resumen: Bovine embryonic stem cells are of potentially big value in transgenic research and studies of lineage commitment and development. Nevertheless, key aspects of the establishment of bovine embryonic stem cells such as the identification of specific pluripotency markers need to be clarified to achieve successful results. Bovine blastocysts were produced in vitro and cultured for 8 days up to the expanded or hatched stage. The trophectoderm, the inner cell mass and its embryonic stem cell-derived lines, all showed a common positive immunocytochemical staining for stage-specific embryonic antigen-4, tumour-rejection antigen gp96 and NANOG proteins. The antigenic profile obtained partially agrees with previous data from bovine and other species. Until a validated pluripotent bovine stem cell marker can be identified, it might be advisable to combine the use of epiblast and trophoblast-specific markers to rule out the presence of early committed trophectoderm cells in bovine embryonic stem cell cultures.
ISSN: 0931-2668
Aparece en las Colecciones:Agroalimentación y Ganadería
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