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Utilice este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/2904


Título: Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification
Autores: Gómez, E.
Rodríguez, A.
Muñoz, M.
Caamaño, José N.
Hidalgo Ordóñez, Carlos O.
Morán, E.
Facal, Nieves
Díez, C.
Palabras Claves: In vitro bovine embryo
Vitrification
BSA
Co-culture
Fecha Edición: May-2008
Editor: Elsevier
Cita Bibliográfica: Gómez, E.; Rodríguez, A.; Muñoz, M. [et. al]. Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification. Theriogenology. 2008; 69 (8): 1013-1021.
Resumen: The aim of this study was to examine the effects of co-culture with Vero cells during the in vitro maturation (IVM) and three culture media, B2+5% fetal calf serum (FCS) on Vero cells, synthetic oviduct fluid (SOF)+5% FCS, and SOF+20 gL(-1) bovine serum albumin (BSA), on the developmental competence of the embryos and their ability to survive vitrification/warming. We also tested the effect of morphological quality and the age of the embryo on its sensitivity to vitrification. The IVM system neither affects the embryo development up to Day 7 nor survival rates after vitrification. The culture of embryos in SOF+FCS and in Vero cells+B2 allowed obtaining more Day 6 and Day 7 blastocysts, and a higher % of Day 7 blastocysts vitrified than culture in SOF+BSA. Contrarily, on Day 8, more blastocysts were vitrified in SOF+BSA than in SOF+FCS. Blastocysts quality affected development after vitrification/warming, and Day 7 embryos showed higher survival rates than their Day 8 counterparts. Day 7 blastocysts produced in Vero cells or in SOF+BSA survived at higher rates than those produced in SOF+FCS at 24 and 48 h after warming. Embryo culture with BSA allows obtaining hatching rates after vitrification/warming higher than those obtained after co-culture with Vero cells in B2 and FCS. Moreover, this system provides hatching rates from Day 8 blastocysts comparable to those obtained on Day 7 in Vero cells. Further studies, including embryo transfer to recipients, are needed to clarify factors affecting the freezability of in vitro produced bovine embryos.
URI: https://ria.asturias.es/RIA/handle/123456789/2904
ISSN: 0093-691X
Aparece en las Colecciones:Agroalimentación y Ganadería
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