Datos del Documento

Por favor, use este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/2905
Título : Structural and functional analysis of virus factories purified from Rabbit vesivirus-infected Vero cells
Autor : Casais, Rosa
González Molleda, Lorenzo
Machín, Ángeles
Barrio, Gloria
García Manso, Alberto
Dalton, Kevin P.
Coto, Ana
Martín Alonso, José M.
Prieto Martín, Miguel
Parra, Francisco
Palabras clave : Caliciviridae
Rabbit vesivirus
Replication complex
Virus factories
Fecha de publicación : oct-2008
Citación : Casais, Rosa; González Molleda, Lorenzo; Machín, Ángeles [et. al]. Structural and functional analysis of virus factories purified from Rabbit vesivirus-infected Vero cells. Virus Research. 2008; 137 (1): 112-121.
Resumen : Rabbit vesivirus infection induces membrane modifications and accumulation of vesicular structures in the cytoplasm of infected Vero cells. Crude RaV replication complexes (RCs) have been purified and their structural and functional properties have been characterized. We show that calnexin, an ER-resident protein, RaV non-structural proteins 2AB-, 2C-, 3A-, 3B- and 3CD-like as well as viral RNAs co-localize within membranous structures which are able to replicate the endogenous RNA templates. The purified virus factories protected their viral RNA contents from microccocal nuclease degradation and were inaccessible to exogenously added synthetic transcripts. In addition, we have shown that RCs can be used to investigate uridylylation of native endogenous VPg. In contrast to the observation that the virus factories were inaccessible to RNAs, RCs were accessible to added recombinant VPg which was subsequently nucleotidylylated. Nevertheless no elongation of an RNA chain attached to native or recombinant VPg could be demonstrated.
URI : http://ria.asturias.es/RIA/handle/123456789/2905
ISSN : 0168-1702
Aparece en las colecciones: Agroalimentación y Ganadería
Open Access DRIVERset

Archivos en este documento:
Fichero Tamaño Formato  
Archivo.pdf1.74 MBAdobe PDFVer/Abrir
Mostrar el registro Completo

Ver estadísticas del documento

Este documento está sujeto a una licencia Creative Commons: Licencia Creative Commons Creative Commons