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Utilice este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/2908


Título: Gene expression in early expanded parthenogenetic and in vitro fertilized bovine blastocysts.
Autores: Gómez, E.
Caamaño, José N.
Bermejo-Álvarez, P.
Díez, C.
Muñoz, M.
Martín, D.
Carrocera, S.
Gutiérrez-Adán, A.
Palabras Claves: Bovine
Embryo
Gene expression
Parthenote
Fecha Edición: Dic-2009
Editor: Japanese Society of Animal Reproduction
Cita Bibliográfica: Gómez, E.; Caamaño, J. N.; Bermejo-Álvarez, P. [et. al]. Gene expression in early expanded parthenogenetic and in vitro fertilized bovine blastocysts. Journal of Reproduction and Development. 2009; 55 (6): 607-614.
Resumen: Mammalian oocytes can undergo artificial parthenogenesis in vitro and develop to the blastocyst stage. In this study, using real-time PCR, we analyzed the expression of genes representative of essential events in development. In vitro matured oocytes were either fertilized or activated with ionomycin + 6-DMAP and cultured in simple medium. The pluripotency-related gene Oct3/4 was downregulated in parthenotes, while the de novo methylation DNMT3A gene was unchanged. Among the pregnancy recognition genes, IFN-t was upregulated, PGRMC1 was downregulated and PLAC8 was unchanged in parthenotes. Among the metabolism genes, SLC2A1 was downregulated, while AKR1B1, COX2, H6PD and TXN were upregulated in parthenotes; there was no difference in SLC2A5. Among the genes involved in compaction/blastulation, GJA1 expression increased in parthenotes, but no differences were detected within ATP1A1 and CDH1. Expression of p66(shc) and the Bax/Bcl2 ratio were higher in parthenotes, and there was no difference in p53. Parthenotes and embryos may differ in the way they stimulate apoptosis, with a preponderant role for p66(shc) within parthenotes. Differentially affected functions may also include pluripotency, de novo methylation and early embryonic signalling.
URI: https://ria.asturias.es/RIA/handle/123456789/2908
ISSN: 0916-8818
Aparece en las Colecciones:Agroalimentación y Ganadería
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