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Utilice este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/527


Título: Macromolecular source as dependent on osmotic pressure and water source: effects on bovine in vitro embryo development and quality
Autores: Duque, Paloma
Hidalgo Ordóñez, Carlos O.
Gómez Piñeiro, Enrique
Pintado, Belén
Facal, Nieves
Díez Monforte, Carmen
Palabras Claves: In vitro produced bovine embryo
Osmolarity
Water quality
Protein
Cell number
Fecha Edición: 2003
Editor: Elsevier
Cita Bibliográfica: Duque, Paloma; Hidalgo, Carlos O.; Gómez, Enrique; Pintado, Belén; Facal, Nieves; Díez, Carmen. Macromolecular source as dependent on osmotic pressure and water source: effects on bovine in vitro embryo development and quality. Reproduction Nutrition Development. 2003; 43: 487-496.
Resumen: This study evaluated the protective effect of protein, as dependent on osmolarity, and the quality of water sources used to prepare embryo culture media. In Experiment 1, two concentrations of NaCl were used to obtain culture media with normal (280 mOSM) and low (245 mOSM) osmolarity, each supplemented with either bovine serum albumin (BSA) or polyvinyl alcohol (PVA). Low osmolarity improved blastocyst rates in the presence of BSA (P < 0.01) and tended to do it in medium containing PVA (P < 0.07). Furthermore, low osmolarity allowed PVA to increase inner cell mass (ICM) numbers and ICM/total cell rate (P < 0.05), while trophectoderm (TE) and total cell counts tended to decrease (P < 0.08). In Experiment 2, culture media were prepared with two water sources (Milli-Q and Sigma-W3500-) in combination with BSA or PVA. Both water sources yielded similar embryo development rates, but in the presence of BSA,Milli-Q water produced embryos with increased ICM/total cells rates (P < 0.05). On the contrary, Sigma water tended to increase trophectoderm cell counts (P < 0.08). In conclusion, the present study showed that low osmolarity is beneficial to embryo development and combinations of macromolecule and osmolarity influence trophectoderm differentiation. Both Milli-Q and Sigma supported embryo development at comparable rates, although in the presence of BSA, blastocysts obtained in the medium prepared with Milli-Q water had superior quality in terms of ICM/total cells rates.
URI: https://ria.asturias.es/RIA/handle/123456789/527
ISBN: 0926-5287
Aparece en las Colecciones:Agroalimentación y Ganadería
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