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Título : Effects of acetoacetate and d-β-hydroxybutyrate on bovine in vitro embryo development in serum-free medium
Autor : Gómez, Enrique
Duque, Paloma
Díaz, Elena
Facal, Nieves
Antolín, Isaac
Hidalgo Ordóñez, Carlos O.
Díez Fernández, Carmen
Palabras clave : Ketone body
In vitro
Fecha de publicación : 15-mar-2002
Editorial : Elsevier Science Inc.
Citación : Gómez, Enrique; Duque, Paloma; Díaz, Elena; Facal, Nieves; Antolín, Isaac; Hidalgo, Carlos; Díez, Carmen. Effects of acetoacetate and d-β-hydroxybutyrate on bovine in vitro embryo development in serum-free medium. Theriogenology. 2002; 57 (5): 1551-1562
Resumen : It is known that the ketone bodies acetoacetate and d-β-hydroxybutyrate can be metabolized by the early bovine embryo for in vitro development. In the present work, we report experiments leading to the culture of bovine embryos in the absence of serum. In vitro-produced bovine zygotes were cultured in modified synthetic oviduct fluid medium supplemented with acetoacetate derivatives, acetoacetate and d-β-hydroxybutyrate. Acetoacetate and its derivatives prevented blastocysts from forming in the absence of serum during the whole culture period. However, from Days 6 to 8 of culture in the absence of serum, acetoacetate did not affect development as compared to controls containing lactate and pyruvate or no substrate. Interestingly, d-β-hydroxybutyrate stimulated blastocyst and expansion development, and allowed lipid mobilization. In feeder cells coculture, embryos produced with d-β-hydroxybutyrate showed improved hatching. Embryos cultured in d-β-hydroxybutyrate were viable upon transfer to recipients, although no pregnancies were confirmed later by ultrasonic scanning. The protective effect of serum upon embryos cultured in medium containing acetoacetate is apparently not required in the presence of d-β-hydroxybutyrate.
URI : http://ria.asturias.es/RIA/handle/123456789/724
ISSN : 0093-691X
Aparece en las colecciones: Agroalimentación y Ganadería
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