Ir a la página de inicio del Gobierno del Principado de Asturias

Datos del Documento

Utilice este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/724


Título: Effects of acetoacetate and d-β-hydroxybutyrate on bovine in vitro embryo development in serum-free medium
Autores: Gómez, Enrique
Duque, Paloma
Díaz, Elena
Facal, Nieves
Antolín, Isaac
Hidalgo Ordóñez, Carlos O.
Díez Fernández, Carmen
Palabras Claves: Ketone body
In vitro
Culture
Bovine
Embryo
Fecha Edición: 15-Mar-2002
Editor: Elsevier Science Inc.
Cita Bibliográfica: Gómez, Enrique; Duque, Paloma; Díaz, Elena; Facal, Nieves; Antolín, Isaac; Hidalgo, Carlos; Díez, Carmen. Effects of acetoacetate and d-β-hydroxybutyrate on bovine in vitro embryo development in serum-free medium. Theriogenology. 2002; 57 (5): 1551-1562
Resumen: It is known that the ketone bodies acetoacetate and d-β-hydroxybutyrate can be metabolized by the early bovine embryo for in vitro development. In the present work, we report experiments leading to the culture of bovine embryos in the absence of serum. In vitro-produced bovine zygotes were cultured in modified synthetic oviduct fluid medium supplemented with acetoacetate derivatives, acetoacetate and d-β-hydroxybutyrate. Acetoacetate and its derivatives prevented blastocysts from forming in the absence of serum during the whole culture period. However, from Days 6 to 8 of culture in the absence of serum, acetoacetate did not affect development as compared to controls containing lactate and pyruvate or no substrate. Interestingly, d-β-hydroxybutyrate stimulated blastocyst and expansion development, and allowed lipid mobilization. In feeder cells coculture, embryos produced with d-β-hydroxybutyrate showed improved hatching. Embryos cultured in d-β-hydroxybutyrate were viable upon transfer to recipients, although no pregnancies were confirmed later by ultrasonic scanning. The protective effect of serum upon embryos cultured in medium containing acetoacetate is apparently not required in the presence of d-β-hydroxybutyrate.
URI: https://ria.asturias.es/RIA/handle/123456789/724
ISSN: 0093-691X
Aparece en las Colecciones:Agroalimentación y Ganadería
Open Access DRIVERset

Archivos en este documento:



Archivo TamañoFormato
Archivo.pdf169,58 kBAdobe PDFVer/Abrir



logo

Todos los documentos en RIA están protegidos por derechos de autor.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - Contacto