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Título: Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability
Autores: Díez Fernández, Carmen
Duque, Paloma
Gómez, Enrique
Hidalgo Ordóñez, Carlos O.
Tamargo Miguel, Carolina
Rodríguez, Aida
Fernández, Lina
Varga, Santiago
Fernández, Alba
Facal, Nieves
Carbajo, Maite
Palabras Claves: Vitrification
Meiotic arrest
Fecha Edición: 15-Jul-2005
Editor: Elsevier Science Inc.
Cita Bibliográfica: Díez, Carmen; Duque, Paloma; Gómez, Enrique; Hidalgo, Carlos O.; Tamargo, Carolina; Rodríguez, Aida [et. al]. Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability. Theriogenology. 2005; 64 (2): 317-333.
Resumen: The nuclear stage at which oocytes are cryopreserved influences further development ability and cryopreservation affects ultrastructure of both cumulus cells and the oocyte. In this work, we analyze the effects of vitrification at different nuclear and cytoplasmic maturation stages on the oocyte ultrastructure and developmental ability. Culture in TCM199 + PVA with roscovitine 25 M during 24 h led to meiotic arrest (MA) in cumulus-oocyte complexes (COCs), while permissive in vitro maturation (IVM) was performed in TCM199, 10% FCS, FSH-LH and 17b-estradiol for 24 h. Oocytes were vitrified using the open pulled straw method (OPS) with minor modifications. Fresh and vitrified/warmed COCs were fixed as immature, after IVM, after meiotic arrest (MA) and after MA + IVM. Vitrification combined with MA followed by IVM produced the highest rates of degeneration, regardless of the vitrification time. As a consequence, lower proportions of embryos cleaved in these groups, although differences were eliminated at the five–eight cell stage. Development rates up to day 8 were similar in all experimental groups, being significantly lower than those in fresh controls. Only oocytes vitrified after IVM were able to give blastociysts. The morphological alterations observed can be responsible for compromised development. More research is needed to explain the low survival rates of the bovine oocyte after vitrification and warming.
ISSN: 0093-691X
Aparece en las Colecciones:Agroalimentación y Ganadería
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