Datos del Documento

Por favor, use este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/9345
Título : Apoptosis-independent poor morphology of bovine embryos produced by multiple ovulation
Autor : Ikeda, S.
Prendes, J.
Alonso Montes, C.
Rodríguez, A.
Díez, C.
Kitagawa, M.
Imai, H.
Gómez E.
Palabras clave : Ovulación múltiple
Transferencia de embriones
Progrma MOET
Fecha de publicación : 2006
Editorial : Blackwell Verlag
Citación : Ikeda, S...[et al.]. Apoptosis-independent poor morphology of bovine embryos produced by multiple ovulation. Reproduction in Domestic Animals. 2006 ; 41 : 383-385
Resumen : In multiple ovulation and embryo transfer (MOET) programmes in cattle, a considerable number of morphologically poor-quality embryos continue to be produced; this is one of the limiting factors of the technique. Apoptosis has often been implicated in developmental arrest and fragmentation; these are regarded as poor traits of embryonic quality in mammalian pre-implantation embryos. In the present study, apoptosis was assessed in morphologically poor-quality embryos in comparison with good-quality embryos that were recovered from a MOET programme. Retarded embryos (two to 16 cell stage), morulae with severe fragmentation and morphologically goodquality morulae recovered from superstimulated cows at day 7 post-insemination were subjected to TdT-mediated dUTP nick-end labelling (TUNEL) and Hoechst staining. Cell nuclei that showed both TUNEL staining and apoptotic morphology were considered to be apoptotic. Apoptotic index (AI) was calculated as the percentage of apoptotic cells per embryo. Fifteen of 17 retarded embryos and 10 of 15 morphologically poor-quality morulae did not show signs of apoptosis. The mean AIs in the morphologically poor-quality embryos (two to 16 cell stage, 2.2%; poor morulae, 1.3%) were as low as that in the good-quality embryos (2.9%). These results suggest that another mode of developmental arrest and/or fragmentation that is independent of apoptosis occurs in morphologically poor-quality embryos recovered from MOET programmes
URI : http://ria.asturias.es/RIA/handle/123456789/9345
ISSN : ISSN 0936-6768
Aparece en las colecciones: Agroalimentación y Ganadería
Open Access DRIVERset

Archivos en este documento:
Fichero Tamaño Formato  
Archivo.pdf522.91 kBAdobe PDFVer/Abrir
Mostrar el registro Completo

Ver estadísticas del documento

Este documento está sujeto a una licencia Creative Commons: Licencia Creative Commons Creative Commons