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Utilice este identificador para citar o enlazar este documento: http://ria.asturias.es/RIA/handle/123456789/7727


Título: Low-level predation by lytic phage phiIPLA-RODI promotes biofilm formation and triggers the stringent response in Staphylococcus aureus.
Autores: Fernández, Lucía
González, Silvia
Campelo, Ana Belén
Martínez, Beatriz
Rodríguez, Ana
García, Pilar
Palabras Claves: Staphylococcus aureus
Biofilms
Bacteriófagos
Fecha Edición: 19-Ene-2017
Editor: Nature Publishing Group
Cita Bibliográfica: Fernández L, González S, Campelo AB, Martínez B, Rodríguez A, García P. Low-level predation by lytic phage phiIPLA-RODI promotes biofilm formation and triggers the stringent response in Staphylococcus aureus. Sci Rep. 2017;7:40965.
Resumen: An important lesson from the war on pathogenic bacteria has been the need to understand the physiological responses and evolution of natural microbial communities. Bacterial populations in the environment are generally forming biofilms subject to some level of phage predation. These multicellular communities are notoriously resistant to antimicrobials and, consequently, very difficult to eradicate. This has sparked the search for new therapeutic alternatives, including phage therapy. This study demonstrates that S. aureus biofilms formed in the presence of a non-lethal dose of phage phiIPLA-RODI exhibit a unique physiological state that could potentially benefit both the host and the predator. Thus, biofilms formed under phage pressure are thicker and have a greater DNA content. Also, the virus-infected biofilm displayed major transcriptional differences compared to an untreated control. Significantly, RNA-seq data revealed activation of the stringent response, which could slow down the advance of the bacteriophage within the biofilm. The end result would be an equilibrium that would help bacterial cells to withstand environmental challenges, while maintaining a reservoir of sensitive bacterial cells available to the phage upon reactivation of the dormant carrier population.
URI: http://ria.asturias.es/RIA/handle/123456789/7727
ISSN: 2045-2322
Aparece en las Colecciones:Agroalimentación y Ganadería

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