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Por favor, use este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/10905
Título : Cancer-associated fibroblasts affect breast cancer cell gene expression, invasion and angiogenesis
Autor : Eiro, Noemi
González, Lucía
Matiinez-Ordoñez, Anxo
Fernández-García, Belén
González, Lu
Cid, Sandra
Dominguez, Francisco
Pérez-Fernandez, Román
Vizoso, Francisco J.
Palabras clave : Angiogenesis
Breast cancer
Cancer-associated fibroblasts
Tumor invasion
Fecha de publicación : mar-2018
Citación : Eiro, N., González, L., Martínez-Ordoñez, A. et al. Cell Oncol. (2018) 41: 369. https://doi.org/10.1007/s13402-018-0371-y
Resumen : Purpose It has been reported that stromal cell features may affect the clinical outcome of breast cancer patients. Cancer associated fibroblasts (CAFs) represent one of the most abundant cell types within the breast cancer stroma. Here, we aimed to explore the influence of CAFs on breast cancer gene expression, as well as on invasion and angiogenesis. Methods qRT-PCR was used to evaluate the expression of several cancer progression related genes (S100A4, TGFβ, FGF2, FGF7, PDGFA, PDGFB, VEGFA, IL6, IL8, uPA, MMP2, MMP9, MMP11 and TIMP1) in the human breast cancer-derived cell lines MCF-7 and MDA-MB-231, before and after co-culture with CAFs. Stromal mononuclear inflammatory cell (MIC) MMP11 expression was used to stratify primary tumors. In addition, we assessed the in vitro effects of CAFs on both MDA-MB-231 breast cancer cell invasion and endothelial cell (HUVEC) tube formation. Results We found that the expression levels of most of the genes tested were significantly increased in both breast cancer-derived cell lines after co-culture with CAFs from either MMP11+ or MMP11- MIC tumors. IL6 and IL8 showed an increased expression in both cancer-derived cell lines after co-culture with CAFs from MMP11+ MIC tumors. We also found that the invasive and angiogenic capacities of, respectively, MDA-MB-231 and HUVEC cells were increased after co-culture with CAFs, especially those from MMP11+ MIC tumors. Conclusions Our data indicate that tumor-derived CAFs can induce up-regulation of genes involved in breast cancer progression. Our data additionally indicate that CAFs, especially those derived from MMP11+ MIC tumors, can promote breast cancer cell invasion and angiogenesis.
URI : https://ria.asturias.es/RIA/handle/123456789/10905
ISSN : 2211-3436
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