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Título: Fibulin-5 downregulates Ki-67 and inhibits proliferation and invasion of breast cancer cells
Autores: Mohamedi Munárriz, Yamina
Fontanil, Tania
Solares, Laura
García Suárez, Olivia
García Piqueras, Jorge
Vega, Jose Antonio
Cal, Santiago
Obaya, Álavaro Jesús
Palabras Claves: Molecular biology
Fecha Edición: 17-Feb-2016
Editor: Spandidos Publications
Cita Bibliográfica: Mohamedi Y, Fontanil T, Solares L, Garcia-Suarez O, Garcia-Piqueras J, Vega JA, Cal S, Obaya AJ: Fibulin-5 downregulates Ki-67 and inhibits proliferation and invasion of breast cancer cells. International journal of oncology 2016;48:1447-1456.
Resumen: Fibulins not only function as molecular bridges within the cellular microenvironment but also influence cell behavior. Thus, fibulins may contribute to create a permissive microenvironment for tumor growth but can also stimulate different mechanisms that may impede tumor progression. This is the case with Fibulin-5, which has been shown to display both tumor-promoting and tumor-protective functions by mechanisms that are not totally defined. We show new evidence on the tumor-protective functions displayed by Fibulin-5 in MCF-7, T47D and MDA-MB-231 breast cancer cells including the inhibition of invasion and proliferation capacity and hampering the ability to form mammospheres. Reduction in the level of phosphorylation of Ser residues involved in the nuclear translocation of β-catenin may underlie these antitumor effects. We also found that Fibulin-5 reduces the level of expression of Ki-67, a nuclear protein associated with cell proliferation. Moreover, reduction in Fibulin-5 expression corresponds to an increase of Ki-67 detection in breast tissue samples. Overall, our data provide new insights into the influence of Fibulin-5 to modify breast cancer cell behavior and contribute to better understand the connections between fibulins and cancer.
Descripción : In the present study, we have employed well-known human breast cancer cell models to overexpress Fibulin-5 and to investigate changes in cell phenotype using different cell-based approaches including cell proliferation, migration and invasion assays, as well as mammosphere formation. Overall, our data suggest that Fibulin-5 induces antitumor effects by suppression of β-catenin phosphorylation. Immunohistochemical analysis of tumor samples of breast cancer patients indicated that a high Fibulin-5 expression level is concomitant with a low expression of the proliferative marker Ki-67, which suggest that Fibulin-5 may influence breast cancer cell proliferation.
ISSN: 1019-6439
Aparece en las Colecciones:Bioquímica
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