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Título : Biological differences between in vitro produced bovine embryos and parthenotes
Autor : Gómez, Enrique
Gutiérrez-Adán, Alfonso
Díez, Carmen
Bermejo-Alvarez, Pablo
Múñoz, Marta
Rodríguez, Aida
Otero, Jesús
Alvarez-Viejo, María
Martín, David
Carrocera, Susana
Caamaño, José Néstor
Palabras clave : Trofectodermo (TE)
Apoptosis
Necrosis
Embriones bovinos
Fecha de publicación : 2009
Editorial : BioScientifica
Citación : Gómez, E... [et al.]. Biological differences between in vitro produced bovine embryos and parthenotes. Reproduction. 2009 ; 137 : 285-295
Resumen : Parthenotes may represent an alternate ethical source of stem cells, once biological differences between parthenotes and embryos can be understood. In this study, we analyzed development, trophectoderm (TE) differentiation, apoptosis/necrosis, and ploidy in parthenotes and in vitro produced bovine embryos. Subsequently, using real-time PCR, we analyzed the expression of genes expected to underlie the observed differences at the blastocyst stage. In vitro matured oocytes were either fertilized or activated with ionomycin C6-DMAP and cultured in simple medium. Parthenotes showed enhanced blastocyst development and diploidy and reduced TE cell counts. Apoptotic and necrotic indexes did not vary, but parthenotes evidenced a higher relative proportion of apoptotic cells between inner cell mass and TE. The pluripotence-related POU5F1 and the methylation DNMT3A genes were downregulated in parthenotes. Among pregnancy recognition genes, TP-1 was upregulated in parthenotes, while PGRMC1 and PLAC8 did not change. Expression of p66shc and BAX/BCL2 ratio were higher, and p53 lower, in parthenotes. Among metabolism genes, SLC2A1 was downregulated, while AKR1B1, PTGS2, H6PD, and TXN were upregulated in parthenotes, and SLC2A5 did not differ. Among genes involved in compaction/blastulation, GJA1 was downregulated in parthenotes, but no differences were detected within ATP1A1 and CDH1.Within parthenotes, the expression levels of SLC2A1, TP-1, and H6PD, and possibly AKR1B1, resemble patterns described in female embryos. The pro-apoptotic profile is more pronounced in parthenotes than in embryos, which may differ in their way to channel apoptotic stimuli, through p66shc and p53 respectively, and in their mechanisms to control pluripotency and de novo methylation.
URI : https://ria.asturias.es/RIA/handle/123456789/11106
ISSN : 1470-1626
Aparece en las colecciones: Agroalimentación y Ganadería
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