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Por favor, use este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/12585
Título : A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients
Autor : Rodríguez Carrio, Javier
Carrillo López, Natalia
Ulloa, Catalina
Seijo, Mariana
Rodríguez García, Minerva
Rodríguez Suárez, Carmen
Díaz Corte, Carmen
Cannata Andía, Jorge B
Suárez, Ana
Dusso, Adriana S
Fecha de publicación : 13-sep-2019
Citación : Rodríguez-Carrio J, Carrillo-López N, Ulloa C, Seijo M, Rodríguez-García M, Rodríguez-Suárez C, Díaz-Corte C, Cannata-Andía JB, Suárez A, Dusso AS. A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients. Sci Rep. 2019 Sep 13;9:13230. doi: 10.1038/s41598-019-49429-x. PMID: 31519925
Resumen : Inflammation is central to chronic kidney disease (CKD) pathogenesis and vascular outcomes, but the exact players remain unidentified. Since low density granulocytes (LDGs) are emerging mediators in inflammatory conditions, we aimed to evaluate whether LDGs may be altered in CKD and related to clinical outcomes as biomarkers. To his end, LDGs subsets were measured in peripheral blood by flow cytometry and confocal microscopy in 33 CKD patients undergoing peritoneal dialysis and 15 healthy controls (HC). Analyses were replicated in an additional cohort. DEF3 (marker of early granulopoiesis) gene expression on PBMCs was quantified by qPCR. Total CD15+ LDGs and both CD14lowCD16+ and CD14-CD16- subsets were expanded in CKD. The relative frequency of the CD14-CD16- subpopulation was higher among the CD15+ pool in CKD. This alteration was stable over-time. The increased CD14-CD16-CD15+ paralleled Kauppila scores and DEF3 expression, whereas no association was found with CD14lowCD16+ CD15+. Both subsets differed in their CD11b, CD10, CD35, CD31, CD62L, IFNAR1 and CD68 expression, FSC/SSC features and nuclear morphology, pointing to different origins and maturation status. In conclusion, LDGs were expanded in CKD showing a skewed distribution towards a CD14-CD16-CD15+ enrichment, in association with vascular calcification. DEF3 expression in PBMC can be a marker of LDG expansion.
URI : https://ria.asturias.es/RIA/handle/123456789/12585
ISSN : 2045-2322
Aparece en las colecciones: Open Access DRIVERset

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