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Utilice este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/12845


Título: Correlation of Micro-Computed Tomography Assessment of Valvular Mineralisation with Histopathological and Immunohistochemical Features of Calcific Aortic Valve Disease
Autores: Solache-Berrocal, Guillermo
Barral-Varela, Ana María
Areces-Rodríguez, Sheila
Junco-Vicente, Alejandro
Vallina-Álvarez, Aitana
Corte-Torres, María Daniela
Valdivielso, José Manuel
Llosa, Juan Carlos
Morís, César
Martín, María
Rodríguez, Isabel
Palabras Claves: calcific aortic valve disease
aortic stenosis
valvular calcification
micro-computed tomography;
histology
immunohistochemistry
macrophages
exosomes
cardiology
Fecha Edición: 21-Dic-2019
Editor: MDPI
Cita Bibliográfica: J. Clin. Med. 2020; 9(1),29
Resumen: Aortic valve stenosis is a serious disease with increasing prevalence in developed countries. Research aimed at uncovering the molecular mechanisms behind its main cause, aortic valve calcification, is thus crucial for the development of future therapies. It is frequently difficult to measure the extent of mineralisation in soft tissues and some methods require the destruction of the sample. Micro-computed tomography (µCT), a non-destructive technique, was used to quantify the density and volume of calcium deposits on cusps from 57 explanted aortic valves. Conventional and immunostaining techniques were used to characterise valve tissue degeneration and the inflammatory and osteogenic stage with several markers. Although most of the analysed cusps came from severe stenosis patients, the µCT parameter bone volume/tissue volume ratio distinguished several degrees of mineralisation that correlated with the degree of structural change in the tissue and the amount of macrophage infiltration as determined by CD68 immunohistochemistry. Interestingly, exosomal markers CD63 and Alix co-localised with macrophage infiltration surrounding calcium deposits, suggesting that those vesicles could be produced at least in part by these immune cells. In conclusion, we have shown that the ex vivo assessment of aortic valve mineralisation with µCT reflects the molecular and cellular changes in pathological valves during progression towards stenosis. Thus, our results give additional validity to quantitative μCT as a convenient laboratory tool for basic research on this type of cardiovascular calcification.
URI: https://ria.asturias.es/RIA/handle/123456789/12845
ISSN: 2077-0383
Aparece en las Colecciones:Sanidad

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