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Utilice este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/2172


Título: Ultrastructure and Development of Vitrified/Warmed Bovine Oocytes Matured with 9-cis Retinoic Acid
Autores: Rodríguez, Aida
Gómez, Enrique
Antolín, Isaac
Duque, Paloma
Hidalgo Ordóñez, Carlos O.
Alonso, Cristina
Tamargo, Carolina
Fernández, Lina
Carbajo, Maite
Facal, Nieves
Caamaño, José N.
Díez, Carmen
Fecha Edición: 2006
Editor: Rodríguez, Aida; Gómez, Enrique; Antolín, Isaac; Duque, Paloma; Hidalgo, Carlos O.; Alonso, Cristina [et. al]. Ultrastructure and Development of Vitrified/Warmed Bovine Oocytes Matured with 9-cis Retinoic Acid. Cell Preservation Technology. 2006; 4 (2): 123-129.
Resumen: In this work we analyze the effects of vitrification on the ultrastructure and developmental ability of bovine oocytes matured in the presence of 9-cis-retinoic acid (RA). Bovine cumulus oocyte complexes (COCs) were matured in a basic medium containing 10% fetal calf serum (FCS), 9-cis-RA or polyvinyl alcohol (PVA). Mature oocytes were vitrified using the Open Pulled Straw method with minor modifications. A group of fresh and vitrified/warmed COCs was fixed for ultrastructural analysis, while the remaining oocytes were fertilized in vitro and cultured. Vitrification of mature oocytes produced high rates of degeneration regardless of the in vitro maturation protocol and low number of cleaved embryos. Development rates up to day 8 were similar between groups undergoing vitrification, which were lower than fresh controls. Morphologic alterations were observed in all groups of vitrified oocytes, although in vitro maturation in the presence of 9-cis-RA combined with vitrification led to higher ultrastructural damage. More research is needed to explain the low survival rates of the bovine oocyte after vitrification and warming.
URI: https://ria.asturias.es/RIA/handle/123456789/2172
ISSN: 1538-344X
Aparece en las Colecciones:Agroalimentación y Ganadería
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