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Utilice este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/428


Título: Identification of an Emergent and Atypical Pseudomonas viridiflava Lineage Causing Bacteriosis in Plants of Agronomic Importance in a Spanish Region
Autores: González Fernández, Ana J.
Rodicio, M. R.
Carmen Mendoza, M.
Palabras Claves: Pseudomonas viridiflava
Fecha Edición: May-2003
Editor: American Society for Microbiology
Cita Bibliográfica: González, Ana J.; Rosario Rodicio, M.; Carmen Mendoza, M. Identification of an Emergent and Atypical Pseudomonas viridiflava Lineage Causing Bacteriosis in Plants of Agronomic Importance in a Spanish Region. Appl. Environ. Microbiol. 2003; 69: 2936-2941.
Resumen: Pseudomonas strains with an atypical LOPAT profile (where LOPAT is a series of determinative tests: L, levan production; O, oxidase production; P, pectinolitic activity; A, arginine dihydrolase production; and T, tobacco hypersensibility) can be regarded as emergent pathogens in the Principality of Asturias (Spain), where they have been causing, since 1999, severe damage in at least three taxonomically unrelated orchard plants of agronomic importance: common bean (Phaseolus vulgaris), kiwifruit (Actinidia deliciosa), and lettuce (Lactuca sativa). These strains are mainly differentiated by production of yellowish mucoid material in hypersucrose medium, used for the levan test, and by a variable pectinolytic activity on different potato varieties. The atypical organisms were identified as Pseudomonas viridiflava based on their 16S rRNA sequences. Among them a certain intraspecies genetic heterogeneity was detected by randomly amplified polymorphic DNA (RAPD) typing. To differentiate between isolates of P. viridiflava and Pseudomonas syringae pathovars, a 16S ribosomal DNA restriction fragment length polymorphism method employing the restriction endonucleases SacI and HinfI was developed. This could be used as a means of reliable species determination after the usual phenotypical characterization, which includes the LOPAT tests.
URI: https://ria.asturias.es/RIA/handle/123456789/428
ISSN: 0099-2240
Aparece en las Colecciones:Agroalimentación y Ganadería
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