Datos del Documento

Por favor, use este identificador para citar o enlazar este documento: https://ria.asturias.es/RIA/handle/123456789/9806
Título : Development and quality of bovine morulae cultured in serum-free medium with retinoic receptor specific agonists
Autor : Gómez, Enrique
Rodríguez, Aida
Múñoz, Marta
Caamaño, José Nestor
Carrocera, Susana
Martín, David
Facal, Nieves
Díez, Carmen
Palabras clave : Apoptosis
Recuentos de células
Embriones producidos in vitro
Paja tirada abierta
Fecha de publicación : 2008
Editorial : CSIRO Publishing
Citación : Gómez, E... [et al.]. Development and quality of bovine morulae cultured in serum-free medium with retinoic receptor specific agonists. Reproduction Fertility and Development. 2008 ; 10 : 884-891
Resumen : Retinoids regulate development and differentiation of the bovine blastocyst in vitro, although the underlying mechanisms remain to be clarified.Achallenge in reproductive biotechnology is the identification of pathways that regulate early embryonic development and their influence on blastocyst differentiation, apoptosis and survival to cryopreservation as traits of embryo quality. The present paper analyses the effects of short-term exposure (24 h) to retinoids on in vitroproduced bovine morulae. Immature cumulus oocyte complexes were in vitro matured and fertilised. Presumptive zygotes were subsequently cultured in modified synthetic oviduct fluid up to Day 6, in which morulae were randomly allocated to the different experimental groups. The treatments consisted of 0.1μm LG100268 (LG; a retinoid X receptor agonist), 0.7μm all-trans retinoic acid (ATRA; a retinoic acid receptor agonist) or no additives. Day 8 blastocyst development was increased in the ATRA-treated group compared with the LG and untreated embryos. In Day 7 embryos, the number of total cells and cells allocated to the trophectoderm were higher in the ATRA-treated group compared with untreated embryos. Apoptosis in the inner cell mass increased after LG treatment, whereas ATRA had no effect. After vitrification and warming, survival and hatching rates of Day 7 blastocysts did not change with retinoid treatment. Within the LGtreated and untreated blastocyst groups, survival and hatching rates were higher for Day 7 than Day 8 embryos; however, Day 8 blastocysts treated withATRA showed improved hatching rates. In conclusion, treatment of morulae withATRA in serum-free medium improves embryo development and quality without increasing the incidence of apoptosis and necrosis.
URI : https://ria.asturias.es/RIA/handle/123456789/9806
ISSN : 1031-3613
Aparece en las colecciones: Agroalimentación y Ganadería
Open Access DRIVERset

Archivos en este documento:
Fichero Tamaño Formato  
Archivo.pdf264.11 kBAdobe PDFVer/Abrir
Mostrar el registro Completo

Ver estadísticas del documento

Este documento está sujeto a una licencia Creative Commons: Licencia Creative Commons Creative Commons